PCR-based detection of alkane monooxygenase genes in the hydrocarbon and crude oil-degrading Acinetobacter strains from petroleum-contaminated soils

Bacterial strains D11, E1 and E2 isolated from petroleum-contaminated soils were found to be members of Acinetobacter genus revealed by 16S rRNA gene sequence analysis phenotypic characteristics. After incubation for 5 days, about 43%, 9% 12% total petroleum hydrocarbons crude oil degraded E2, respectively, determined GC-MS analysis. Moreover, 70% 76% single hydrocarbon hexadecane was the D11 after 3 days short time, while strain 48% pentadecane. By using PCR-based method, sequences shown similarity alkane 1-monooxygenases sp. BUU8 alkM with 93.06% 92.72%, 95.84% sp.826659. The present study biodegradation may provide a good advantage in bioremediation process.